Composite

Part:BBa_K3002232:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + cCA_MutateM_SP20HA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 4325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (hygromycin resistance) and BBa_K3002100 (cCA_MutateM_SP20HA).

References